Clin Res Cardiol 100, Suppl 1, April 2011

P644 - Mechanical stretch of sympathetic neurons increases VEGF expression via a NGF signaling pathway
E. Saygili1, O. R. Rana1, E. Saygili1, D. Hommes1, R. H. G. Schwinger2, N. Marx1, P. Schauerte1
1Medizinische Klinik I - Kardiologie, Pneumologie und Angiologie, Universitätsklinikum Aachen, Aachen; 2Medizinische Klinik II, Kardiologie, Klinikum Weiden, Weiden;
Background: During myocardial infarction (MI) or chronic heart failure (CHF) intracardiac cells are exposed to increased mechanical stretch. Recently, we have shown that mechanical stretch of sympathetic neurons (SN) induces nerve sprouting by up-regulation of nerve growth factor (NGF). It has been reported that NGF also functions as an angiogenic factor, promoting neovascularization and improving cardiac function after MI. Whether these observations are due to a direct NGF function, or whether NGF promotes cellular VEGF expression contributing to neovascularization is rather unknown. Therefore, this study aimed to investigate the expression of VEGF in pure cell cultures of SN exposed to mechanical stretch, as well the role of NGF in promoting cellular VEGF expression.
Methods: Pure cell cultures of sympathetic neurons from the superior cervical ganglia of neonatal Sprangue Dawley rats were exposed to a gradual increase of stretch from 3 up to 13% every 24 hours over 3 days (steps: 3, 7, 13 %). The VEGF expression was determined on mRNA and protein level.
Results: Mechanical stretch significantly increased VEGF mRNA expression (VEGF: control = 1 vs. stretch = 3,5, n=5). Western blot and ELISA experiments could confirm these findings on protein level. This response was time dependent. NGF neutralizing antibodies significantly decreased mechanical stretch induced VEGF expression (VEGF: stretch + NGF neutralizing = 1,7, n=3).
Conclusion: SN respond to mechanical stretch with an up-regulation of VEGF, which in part is mediated by a NGF signaling pathway.
Clin Res Cardiol 100, Suppl 1, April 2011
Zitierung mit Vortrags- oder Posternummer s.o.
DOI 10.1007/s00392-011-1100-y