Clin Res Cardiol 101, Suppl 1, April 2012

P369 - Monocytes and Dendritic Cells of patients with peripheral arterial disease show an ameliorated proinflammatory phenotype over 6 Months parallel to an increased walking distance
 
J. F. Dopheide1, M. Scheer1, V. Obst1, M.-C. Radmacher1, M. Radsak1, T. Gori1, A. Warnholtz1, A. Daiber2, T. Münzel1, C. Espinola-Klein1
 
1II. Medizinische Klinik und Poliklinik, Universitätsmedizin der Johannes-Gutenberg-Universität, Mainz; 2II. Med. Klinik - Labor für Molekulare Kardiologie, Klinikum der Johannes Gutenberg-Universität, Mainz;
 
Background: Atherosclerosis is a disease triggered by diverse exogenous stimuli and sustained by chronic inflammatory processes. Monocytes and Dendritic cells (DC) play a crucial role in regulating chronic inflammatory process.
Methods: Peripheral blood leucocytes of 60 patients with peripheral arterial disease (PAD) with intermittent claudicatio (IC, Fontaine State II a and b) and 30 healthy controls were analysed from whole blood by flow cytometry (FACS-Canto, bd-biosciences). Monocytes and DC were identified by different gating strategies in relation to size and granulation (FSC/ SSC) and surface molecules (CD14/CD45 for monocytes; HLA-DR/ CD11c/CD123 for DC) and analysed for CD14/ CD16 and M-DC8 (Slan) on monocytes, and for CD86, CD80, CD40 and CD83 on DC. Clinical data including CRP, fibrinogen, ankle-brachial-index (ABI) and walking distance were analysed after standard protocol. Follow-up was performed after 6 months.
Results: PAD patients show a significant increased proportion of proinflammatory CD14-CD16++ and M-DC8+ monocytes in comparison to healthy controls (all p<0.001). Myeloid and plasmacytoid DC (mDC, pDC) did not differ between patients and controls. After 6 months, we observed a shift in PAD patients towards a significant increased proportion of antiinflammtory CD14++CD16+ monocytes (p<0.01), and parallely a decrease in proinflammatory CD14-CD16++ and M-DC8+ monocytes (both p<0.05) compared to the beginning of the study. Similarly, mDC of PAD patients had a significant decreased expression of CD86, CD80, CD40 and CD83 (all p<0.05), whereas pDC showed no changes after 6 months. CRP, fibrinogen and ABI did not differ significantly after 6 months. However, the pain free walking distance increased about 250 m (p<0.05).
Conclusions: Peripheral blood monocytes and DC of patients with PAD show a decreased proinflammatory phenotype after 6 months parallel to an increased walking distance. Here, we show in vivo insights in the process of atherogenesis of patients with PAD in the state of intermittent claudication indicating an amelioration of the disease through anti-inflammatory strategies like intensive exercise training.
 
Clin Res Cardiol 101, Suppl 1, April 2012
Zitierung mit Vortrags- oder Posternummer s.o.
DOI 10.1007/s00392-012-1100-6

http://www.abstractserver.de/dgk2012/ft/abstracts/P369.htm